ALBA II Long Beamlines Workshop

14 Nov 2022, 14:15 → 15 Nov 2022, 13:00 Europe/Madrid

Maxwell Auditorium + Zoom (ALBA Synchrotron)

Caterina Biscari, Klaus Attenkofer

ALBA has started planning, designing, prototyping its evolution towards ALBA II, the 4th generation brighter light source. A call for proposals has been launched to prepare scientific cases for 4 possible long beamlines to be installed at ALBA.

This workshop is aimed at presenting the scientific opportunities that ALBA II will bring the user community together with the pre-proposals for these long beamlines.

Monday, 14 November

1 Introduction to the workshop

Speaker: Caterina Biscari

2 Opportunities in imaging

Speaker: Anne Bonnin (PSI)

3 X ray Microscopy in life science

Speaker: Jean Susini (Soleil Synchrotron)

4 X ray Microscopy in material science

Speaker: Xiaojing Huang (Brookhaven National Lab.)

ALBA_2022_xjhuang.pdf

15:50 Break

5 Imaging under extreme conditions

Speaker: Hanns-Peter Liermann (DESY)

P02_2_imaging_extreme_conditions_short.pdf

6 How does a hard X-ray nano probe work

Speaker: Jonathan Wright (ESRF, Grenoble, France)

Hard_Energy_NanoProbe_2022_Wright.pdf

7 How does a soft X-ray nano probe work

Speaker: Alessandra Gianoncelli (Elettra Sincrotrone)

AlbaII_Gianoncelli_final.pdf

8 Introduction to the project

Speaker: Caterina Biscari

Close out.pptx

Introduction to ALBA II.pptx

20:30 Dinner

Bus service is provided from ALBA and EXE Parc Hotel

Tuesday, 15 November

9 Overview of proposals

Speaker: Josep Nicolàs Roman

LongBL_Workshop.pptx

Presentation of preproposals

10 CORUS: a cryo nano bio-imaging beamline for ALBA-II

The complexity of biological systems requires monitoring them at “work” to link molecular processes in the native cellular environment with structural and functional information. Molecular location and quantification in close-to-native conditions, avoiding chemical fixation and dehydration artefacts, are therefore of outmost importance for instance in metallomics, drug delivery, nano-biomedicine and infection, among others. To this aim, we propose CORUS as a cryogenic bio nanoprobe beamline with two complementary end-stations that will allow performing nano X-ray fluorescence (XRF) and phase contrast-based imaging (PCI) in one of them and nano-XANES in the other, in cryopreserved cells, organoids and tissues to respond to current and future societal challenges. NanoImag, the first end-station, will deliver a focal spot of 30 nm at 10, 20 and 27 keV with high flux to allow ppm sensitivity, as well as revealing structural information of the sample by PCI. This end-station will be optimized for the study of endogenous elements (10 keV), sub-optimally tackled at higher excitation energies, but will also be capable of probing the most common exogenous elements used for biomedical and biotechnological applications. Moreover, NanoImag will be complementary to NanoSpec, the second end-station, which will focus on spectroscopic capabilities from 4 to 25.5 keV at cryo with a 50-100 nm focus. CORUS will complete the ALBA-II portfolio enabling multimodal and correlative research with current operational beamlines such as MISTRAL, FAXTOR and MIRAS, but will also be complementary to the cryo-epifluorescence and cryo-3D Structured Illumination microscopes and the cryo-EM facilities. Remarkably, this beamline array would be unique in Europe, and could place ALBA-II into an outstanding position as a powerhouse for correlative multi-technique approaches involving all three cryo-visible light, EM and X-ray imaging.

Speaker: Jose Javier Conesa (CNB-CSIC)

LongBeamlineWorkshopALBA_Nov2022.pdf

11 From mammalian circuits to synapses: correlative multimodal imaging using hard X-rays

Integrating physiology and structure at the neuronal circuit scale can provide a mechanistic understanding on how that circuit works. A correlative multimodal imaging pipeline that combines in vivo 2-photon microscopy (2P), synchrotron X-ray computed tomography with propagation-based phase contrast (SXRT) and serial block-face electron microscopy generates these multimodal maps reliably. In it, SXRT brings subcellular context of multi-mm3 landscapes non-destructively. SXRT also enables a bridging use: 2P and SXRT datasets can be warped at single-cell accuracy, informing on optimal specimen trimming strategies. Finally, this pipeline is compatible with other complementary hard X-ray imaging modalities: X-ray nano-holotomography resolves lateral dendrites of mitral and tufted neurons of known physiological profiles, and X-ray ptychographic tomography could resolve 60% of the synaptic contacts with an 80% precision. Altogether, this approach enables harnessing the resolving power of multiphoton, hard X-ray and volume electron microscopy technologies to create detailed multimodal maps of brain circuits.

Speaker: Carles Bosch Piñol (The Francis Crick Institute, London, UK)

ALBA2_BoschC_online.pptx

12 CoDI, Coherence Diffraction Imaging

CoDI will be the coherence diffraction end station at ALBA II. This station will be intended to investigate materials in strategic sectors for the European Union (biotechnology and agri-food; energy transition and decarbonization; active and healthy ageing; circular economy and sustainable raw materials), which complement ALBA strategic research lines. The main technique at CoDI will be ptychographic-tomography in forward direction, making possible the imaging of thick (< 500 m) nanostructured materials (e.g. nanocatalysts, nanocrystals, nanoparticles) and biological samples. The setup will be compatible with tele-ptychography (a new variant of ptychography that use an analyzer to reconstruct complex wave fronts after the sample, this simplifies in-situ experiments), scanning approaches, near-field ptychograpy, Bragg-CDI and Bragg-ptychography. A tailored setup for tele-ptychography will make CoDI at ALBA II an unique instrument in the world.
CoDI beamline perfectly suits within the societal challenges defined at European level, as well as in the ALBA strategic research lines. This will help to the different national and international academic and industrial community to profit about this highly advanced beamline.
Scientific communities that will benefit from imaging thin and thick samples till 500 m, such as biology, catalysis, electrocatalysis, energy related materials and general material science.

Speaker: Prof. P. Villanueva-Pérez (Universidad de Lund, Sweden)

CoDI_Nov_2022.pdf

13 Probing matter at the nano-to-mesoscale in the real and reciprocal space with chemical and bond-orientation sensitivity

A beamline with two endstations is envisaged. One of the endstations will be devoted to Resonant Soft X-ray Scattering (RSoXS) and the other one to Scanning Transmission X-ray Microscopy (STXM) + ptychography.
Scanning Transmission X-ray Microscopy and Resonant Soft X-ray scattering are complementary techniques to probe the nano-to-mesoscale structure of soft condensed matter, in the real and reciprocal space, respectively. It has been demonstrated that by using variable wavelength X-ray probes around the light element absorption edges of carbon, nitrogen and oxygen, the resonant behavior of chemicals containing these elements can be used as fingerprints of the molecular bonding environment, including the molecular orientation. The unique chemical and orientational sensitivities of both, RSoXS and STXM, arise from the energy- and polarization-dependent X-ray interaction with molecules. While usual non-resonant scattering techniques have difficulty for distinguishing organic materials in a sample because they all have very similar electron densities, due to chemical sensitivity, RSoXS can increase the contrast between these materials orders of magnitude.
RSoXS and STXM techniques can address scientific cases like: organic electronics, liquid crystals, polyelectrolyte solutions and gels, membranes and porous materials, self direct assembly, polymers, nanocomposites, interfacial structures, low Z catalysis, biology, etc. Some examples will be pointed out.

Speaker: Mari Cruz García-Gutiérrez (Instituto de EStructura de la Materia IEM-CSIC)

Garcia-Gutierrez_ALBA II Long Beamlines.pdf

14 Soft x-ray RIXS. A new long beamline at ALBA

The current needs generated by the race towards understanding and tailoring properties of quantum materials and the urgent social demand of greener and/or sustainable energies has boosted the demand of mapping weak excitations in matter. Resonant Inelastic X-ray Scattering, RIXS, is a unique tool that can return full energy and momentum resolved maps of such excitations, and has provided new insights in many different areas, from superconductivity, magnetism and energy harvesting (batteries, data storage,...). There is an exceeding large demand of beam time at RIXS beamlines that cannot be covered by the large scale facilities (ERSF, Diamond, NSLS II, Soleil,…). There is also the need to develop tools that could allow mapping micrometric samples and devices, and perform analysis under flexible variety of external stimuli. These are the goals that the new long beamline aims to offer to the scientific community.

Speakers: Santiago Blanco Canosa (Donostia International Physics Center), Prof. Josep Fontcuberta (Institut de Ciència de Materials de Barcelona. Campus UAB. Bellaterra), Prof. Enrique Garcia Michel (Universidad Atononma Madrid), Dr Adriana Figueroa (Universidad Barcelona), Dr Julio Camarero (Universidad Autonoma de Madrid), Dr Sara Lafuerza (INMA, CSIC-Universidad Zaragoza), Dr Manuel Valvidares (Institut de Ciència de Materials de Barcelona. Campus UAB. Bellaterra), Dr Jordi Fraxedas (ICN2), Dr Gervasi Herranz (ICMAB)

RIXS_ALBA.pdf

15 Discussion: working group Life Science

16 Discussion: working group Materials Science

11:45 Break

17 Close out

Speaker: Klaus Attenkofer

Close out.pdf