Conveners
Session 6 - II ALBA User's Meeting 2015
- Miguel A. G. Aranda (ALBA-CELLS)
Dr
Rafael Molina
(CNIO)
18/06/2015, 10:00
II ALBA User's Meeting
Oral presentations
The enzymatic hydrolysis of DNA phosphodiester bonds has been widely studied, but the chemical reaction has not yet been observed. Here we follow the generation of a DNA double-strand break (DSB) by the Desulfurococcus mobilis homing endonuclease I-DmoI, trapping sequential stages of a two-metal-ion cleavage mechanism. We captured intermediates of the different catalytic steps, and this...
Rafael Junqueira Borges
(IBMB-CSIC, UNESP)
18/06/2015, 10:20
II ALBA User's Meeting
Oral presentations
Phasing your XALOC data with ARCIMBOLDO
Macromolecular Ab Initio phasing, from the native intensities alone with no experimental phase information or previous particular structural knowledge has been the object of a long quest, limited by two main barriers: structure size and data resolution. Beyond the first atomic resolution successes, current approaches have been developed, exploiting...
Prof.
Daniela Uhrikova
(Faculty of Pharmacy, Comenius University in Bratislava)
18/06/2015, 10:40
II ALBA User's Meeting
Oral presentations
DNA polyanion interacts with a dispersion of cationic liposomes forming supramolecular assemblies of regular inner microstructure – lipoplexes. They are studied as delivery vectors for genetic material. Despite the fact that cationic liposomes have been used for transfection, and commercial lipid formulations are available, their efficiency needs to be improved.
We will discuss structural...
