Speakers
Dr
Ibraheem Yousef
(ALBA Synchrotron)Dr
Immaculada Martínez-Rovira
(Centre National de la Recherche Scientifique (CNRS))
Description
Radiotherapy plays a key role in the treatment of cancer. The main limitation is to reach curative doses in the tumour while sparing the surrounding healthy tissue. One strategy to improve the clinical outcome in radiotherapy is to increase the dose effects in the tumour. This can be achieved by using specific nanoparticles (NPs). Numerous studies have shown the enhanced effectiveness of tumour control when NPs were used [1-3]. However, the involved biochemical mechanisms are not yet clear. In addition to a possible dose enhancement, the size similarity of NPs to biological molecules could provide “camouflage” to cellular
barriers, leading to changes in the cellular function and producing cell arrest at radiosensitive phases or
oxidative stress [4,5]. These effects, which could be amplified with a subsequent irradiation, might increase their
anticancer effectiveness. Within this framework, in this study we used F98 glioma rat cells as an *in vitro* model to
disentangle the biochemical changes in the cells induced by x-ray irradiations in combination with NPs. These biochemical processes can be studied by using Fourier transform infrared microspectroscopy
(FTIR). FTIR allows in situ structure determination of the most important biomolecules, and it is extremely useful
to study cell cycle, differentiation and proliferation of cell lines and cell death mode [6-8].
Within this context, F98 glioma cells were irradiated with x-rays in the presence and absence of Gadolinium NPs.
FTIR measurements were performed by using the internal globar source at SESAME synchrotron (Jordan).
Principal Component Analysis (PCA) was performed to show the variances between two different sets of
spectra. Flow cytometry and cell viability assays were also carried out.
Preliminary results are very encouraging. Figure 1 shows the PCA in the sugar and DNA region of the infrared
spectra. The differences in the presence (blue) and absence (red) of Gd NPs are clearly observed for a dose of
10 Gy. PCA reveals also clear differences in the proteins and lipids regions. Biological interpretation is in
progress nowadays. Forthcoming studies will consist in the evaluation of other radiotherapy approaches and
other types of NPs at ALBA MIRAS beamline
References
[1] J.F. Hainfield et al. Phys. Med. Biol. 49 (2004);
[2] J.F. Hainfield et al. J. Pharm. Pharmacol. 60 (2008); [3] C. Alric et al. J. Am. Chem. Soc. 130 (2008);
[4] D. Choudhury et al. Small 1 (2005);
[5] E. E. Connor et al. Nanoscale 5 (2013).
[6] J. Sulé-Suso et al. J. Vibrational Spectroscopy 38 (2005);
[7] L. Buriankova et al. Laser Phys. Lett. 7 (2010);
[8] I. Yousef et al. Analyst 136 (2011);
| Caption (s) - Add figures as attached files (2 fig. max) | Figure 1. PCA in the sugar and DNA region of the infrared spectra of F98 glioma cells for a dose of 10 Gy. Blue points correspond to the presence of Gd NPs and red points to the absence of Gd NPs. |
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Primary author
Dr
Ibraheem Yousef
(ALBA Synchrotron)
Co-authors
Dr
Immaculada Martínez-Rovira
(Centre National de la Recherche Scientifique (CNRS))
Dr
Josep Sulé-Suso
(Keele University)
Dr
Olivier Seksek
(Centre National de la Recherche Scientifique (CNRS))
Dr
Sílvia Gil
(Hospital Parc Taulí)
Dr
Yolanda Prezado
(Centre National de la Recherche Scientifique (CNRS))
